Determination of Luteolin in Celery Seed Extract by HPLC

OBJECTIVE To establish a method for the determination of luteolin in celery seed extract. METHODS The analysis was performed by HPLC with Apollo C₁₈ column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid (30∶70), and the detection wavelength was set at 269 nm. The column temperature was 30 ℃. RESULTS The linear range was 0.50 to 10.0 μg·mL^-1 for luteolin (r=0.999 8). The average recovery of three concentration were 100.2%, 100.1% and 101.2%, RSD were 1.02%, 1.91% and 1.75%. CONCLUSION The method is feasible, accurate and high selectivity for the determination of luteolin in celery seed extract.