Determination of Scopolamine in Datura stramonium by HPLC

OBJECTIVE To establish the method of determination of scopolamine in Datura stramonium. METHODS Kromasil C₁₈(250 mm×4.6 mm, 5 mm) column was employed. Mobile phase was the mixture of acetonitrile and Na3PO4 buffer (contains 0.07 mol·L^-1 Na₃PO₄, 0.017 5 mol·L^-1 SDS, 0.5% H₃PO₄, 0.1% trithylamine)(37∶63). RESULTS A good linearity of scopolamine was achieved in the range of 0.038 1-0.190 5 mg·mL^-1(r=0.999 9). The average recovery of scopolamine was 96.97% and RSD was 0.93%. CONCLUSION This method is simple, sensitive, accurate with good reproducibility, and can be used for the quality control of Datura stramonium.