OBJECTIVE To establish an HPLC-MS/MS method for the determination of O-Nornuciferine in Ziziphi Spinosae Semen and Ziziphi mauritianae Semen.
METHODS Phenomenex Kinetex C18 column(100 mm×2.1 mm, 2.6 μm) was used for separation. The mobile phase consist of methnol, acetonitrile and 0.1% formic acid-H2O(7.5︰7.5︰85). The flow rate was 0.3 mL·min−1. Electrospray positive ion(ESI+) source was used for mass spectrometry detection by multiple reaction monitoring mode.
RESULTS The liner range of calibration curve was 0.00049–0.98 ng(r=0.9992). The RSD of repeatability test was 4.2%. The average recovery was 87.0%, RSD was 1.5%. The limit of detection and quantitation were 0.116 ng·g−1 and 0.386 ng·g−1.
CONCLUSION The method has well specificity, sensitivity and applicable, which provides reference for quality control of Ziziphi Spinosae Semen.
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