Study on Quality Evaluation of Zhuang Medicinal Jasmine Based on UPLC Fingerprint and Multi-component Quantitative Combined with Chemometric Method

OBJECTIVE To establish the UPLC fingerprint of Zhuang medicine Jasmine, and determine the content of 5 chemical components, combined with stoichiometry to evaluate the quality of Jasmine more comprehensively.

METHODS The chromatography was performed on Agilent ZORBAX RR StableBond C₁₈(4.6 mm×150 mm, 3.5 µm) column with acetonitrile-0.1% phosphoric acid solution as mobile phase and gradient elution. The flow rate was 0.3 mL·min⁻¹, the column temperature was 30 ℃, and the detection wavelength was 360 nm. The UPLC fingerprints of Jasmine were established, and the contents of rutin, hypericin, quercetin, kaempferol and oleanolic acid were determined. The whole quality of Jasmine was evaluated based on the peak-to-peak area of the fingerprints combined with stoichiometric analysis.

RESULTS  The similarity of 44 batches of Jasmine fingerprints from different producing areas and batches was greater than 0.980. A total of 22 common peaks were calibrated and the above 5 common peaks were identified. The results of cluster analysis showed that all samples clustered into three categories. A total of 4 principal components were extracted by principal component analysis, and the comprehensive score ranged from −4.32 to 3.20. Pearson correlation analysis showed that the above 5 components and their total content could be used as the evaluation indicators of Jasmine quality. There was no significant difference in the content of kaempferol among the samples from different regions. The contents of rutin, hyperoside and quercetin in the Guangxi group were low as a whole, and the content of oleanolic acid in the Yunnan group was the highest.

CONCLUSION The established method is efficient, simple and stable, which can comprehensively evaluate the overall quality of Zhuang medicinal Jasmine, and provide a scientific basis for improving its quality standards.