Feasibility Study of an in Vitro Pyrogen Detection System for Human-derived Monocyte Cell Line 28SC

OBJECTIVE To investigate the feasibility of in vitro detection of pyrogen by the human-derived monocyte cell line 28SC.

METHODS 28SC was incubated separately with blank control(IMDM) and lipopolysaccharide(LPS), and the changes in the content of the relevant cytokine IL-6 in the incubation system were detected by enzyme-linked immunosorbent assay(ELISA). Stimulate 28SC with calcitriol to establish a 28SC pyrogen detection system, establishing the 28SC pyrogen detection system; investigating the minimum detection limit and linear range of the 28SC in vitro pyrogen detection system.

RESULTS When the concentration of calcitriol was 500 ng·mL⁻¹, the 28SC-IL-1β and TNF-α could detect 0.5 EU·mL⁻¹ for LPS, and did not react to LTA and Zymosan, and the 28SC-IL-6 could detect 0.125 EU·mL⁻¹ for LPS, and the detection limit of the 28SC pyrogen assay system was determined to be 0.5 EU·mL⁻¹.

CONCLUSION This study determines that the concentration of the activator calcitriol in the 28SC pyrogen detection system is 500 ng·mL⁻¹, and the detection indicator is cytokine IL-6.