Analysis of Chemical Constituents in Methanol Extracts of Siraitia Grosvenorii Leaves by UPLC-Q-TOF-MS

OBJECTIVE To establish a rapid qualitative method for ethanol extract from the leaves of Siraitia grosvenorii by using ultra performance liquidchromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS). METHODS Chromatographic column: ACQUITYUPLC UPLC BEH C₁₈(2.1 mm×100 mm, 1.8 μm); injection volume: 5 μL; flow rate: 0.3 mL·min^-1, mobile phase was 0.1% formic acid solution(A)-acetonitrile solution(B) with gradient elution sequence; mass spectrum condition: electrospray ion source(ESI), detection range m/z 50-1 000, positive and negative ions were used for MSE mode acquisition respectively, based on the high-resolution molecular weight and mass spectrum fragment information, combined with database matching, the mass spectrum data were analyzed. RESULTS A total of 64 compounds were characterized from the alcohol extract of Siraitia grosvenorii leaves, including seven flavonoids, fourteen acids, twelve esters, ten triterpenoids, four ketones, four alcohols and thirteen others. CONCLUSION UPLC-Q-TOF-MS provides a simple, efficient and accurate method for the identification of chemical constituents in the alcohol extraction of Siraitia grosvenorii leaves.