Quality evaluation of Coreopsis tinctorial from different producing areas based on HPLC fingerprint and chemometrics

OBJECTIVE To establish HPLC fingerprint of Coreopsis tinctorial, so as to evaluate the quality of Coreopsis tinctorial from different areas by content determination of four components combined with chemometrics. METHODS Elite SinoChrom ODS-BP C₁₈ column(4.6 mm×250 mm, 5 μm) was used, the mobile phase was acetonitrile-0.1% phosphoric acid solution, gradient elution, the flow rate was 0.6 mL·min^-1, the detection wavelength was 295 nm, and the column temperature was 30℃. The fingerprint of Coreopsis tinctorial from different producing areas was established, and the similarity evaluation, cluster analysis(CA), principal component analysis(PCA) and partial least square discriminant analysis(PLS-DA) were carried out. Combined with the projectionvalue of variables(VIP), the main difference components were identified and the content was determined. RESULTS The fingerprints of 12 batches of Coreopsis tinctorial contained 21 common peaks, and the similarity between all samples and the control map was ≥ 0.942. By CA analysis and PCA analysis, 12 batches of Coreopsis tinctorial were grouped into 3 groups. The results of PLS-DA analysis were consistent with those of CA analysis. The contents of four main differential components selected by VIP value were marinoside 40.05-61.25 mg·g^-1, flavanomanthin 7.44-19.82 mg·g^-1, rutin 0.85-2.03 mg·g^-1, chlorogenic acid 2.56-9.73 mg·g^-1, respectively. CONCLUSION The fingerprint analysis and content determination method established in this experiment is reliable and reproducible, which provides a basis for the overall quality evaluation of Coreopsis tinctorial.