Study on Antibacterial in Vitro and Antioxidant Activity of Grifola Frondosa Polysaccharide

OBJECTIVE To study the antibacterial effect and antioxidant activity of Grifola frondosa polysaccharide. METHODS UV spectrophotometry was used to determine the content of polysaccharide, D-anhydroglucose was used as a standard. The antibacterial effect produced by Kirby-Baue method with the bacteria of Staphylococcus aureus, Escherichia coli and Bacillus subtilis. Took the scavenging capacity as index to estimate the endogenous antioxidative activity of DPPH and T-AOC. RESULTS The total polysaccharides in Grifola frondosa extract was 53.82%. Grifola frondosa polysaccharide had a good antibacterial effect on Staphylococcus aureus, Escherichia coli and Bacillus subtilis, the diameter of antibacterial were 13, 11, 10 mm. With the concentration between 0.05~10 mg·mL^-1, Grifola frondosa polysaccharide had a good scavenging activity by DPPH, when the concentration above 0.5 mg·mL^-1, showed the total antioxidant activity. CONCLUSION The polysaccharides in Grifola frondosa has certain antibacterial effect and antioxidant activity.