Study on Quality Control Method of Jianwei Mixture

OBJECTIVE To establish the quality control method of Jianwei mixture. METHODS Thin layer chromatography(TLC) was used to identify Glycyrrhizae Radix et Rhizoma, Coptidis Rhizoma, stir-baked Aurantii Fructus in bran. Determination of naringin content by HPLC. The mobile phase for determining naringin was methanol-0.2% phosphoric acid(40:60), the UV detection wavelength was 283 nm, Diamonsil C₁₈(250 mm×4.6 mm, 5 μm) column was used for the determination, and the flow rate was 1.0 mL·min^-1, the column temperature was 30℃ and the injection volume was 5 μL. RESULTS TLC showed clear spots and good resolution. The linearity of naringin was in the range of 0.005 33-0.085 23 mg·mL^-1, the average recovery of naringin was 99.42%, RSD was 0.76%. CONCLUSION The method is accurate, reliable and exclusive, it can be used for the quality control of Jianwei mixture.