Content Determination of Quercitrin in Naoxinqing Crude Drug and Tablets by HPLC

OBJECTIVE To establish a method of determine the content of quercitrin in Naoxinqing crude drug and tablets by HPLC. METHODS The samples were separated on an Agilent Eclipse Plus C₁₈ column (250 mm×4.6 mm, 5 μm), by a gradient elution using the mobile phase of acetonitrile (A)-0.1% phosphoric acid (B) (0~40 min, 7%→ 25%A; 40~60 min, 25%→ 50%A) at a flow rate of 1.0 mL·min^-1. The detection wavelength was set on 360 nm and the column temperature was set at 30℃. RESULTS A good linearity was observed for quercitrin in the range of 19.4-775.7 μg·mL^-1(r=0.999 9). The average recoveries(n=6) of Naoxinqing crude drug and tablets were 100.07% and 100.16% with the RSD of 1.88% and 1.45%, respectively. CONCLUSION The method is simple, accurate, reproducible, and applicable for the content determination of quercitrin in Naoxinqing crude drug and tablets.