Establishment of HPLC Fingerprint for Populi Flos

OBJECTIVE To control the quality of populi Flos, HPLC fingerprint of populi Flos was established. METHODS The samples were analyzed by HPLC, which included 10 batches of different places of populi Flos. RESULTS HPLC fingerprint was established, confirmed the HPLC condition. Unitary C₁₈ column (4.6 mm×250 mm, 5 µm) was used, the mobile phase was acetonitrile-0.1%formic acid, and the wavelength was 290 nm. Through the construction of HPLC fingerprint, the 12 peaks were established and the similarity of 10 batches of populi Flos were about 0.9-1.0, which clustered into 2 categories. CONCLUSION The methodological evaluation showes that this method has a good precision, repeatability and reproducibility.