Simultaneous Determiation of Tanshinol, Protocatechualdehyde, Salvianolic Acid B, Ferulic Acid and Puerarin in Tongmai Particles by HPLC

OBJECTIVE To estahlish an HPLC method for the simultaneous determination of tanshinol, protocatechualdehyde, salvianolic acid B, ferulic acid and puerarin in Tongmai particle by HPLC. METHODS The Welch Ultimate XD-C₁₈ colunm(4.6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-0.1% trifluoroacetic acid in gradient mode. The flow rate was 1.0 mL·min^-1 with UV detection wavelength at 282 nm and 305 nm. The column temperature was maintained at 35℃. RESULTS The linear ranges of tanshinol, salvianolic acid B, protocatechualdehyde, puerarin and ferulic acid were in the range of 3.117-62.33 μg·mL^-1(r=0.998 7), 4.044-80.88 μg·mL^-1(r=0.998 5), 1.280-25.60 μg·mL^-1 (r=0.997 9), 7.964-159.3 μg·mL^-1(r=0.992 8), 1.980-39.60 μg·mL^-1(r=0.999 1) with average recoveries of 101.6%(RSD=1.62%), 99.7%(RSD=1.76%), 97.4%(RSD=1.19%), 99.9%(RSD=1.52%), 102.2%(RSD=1.56%), respectively. CONCLUSION This method is simple, fast and accurate, which is suitable for quality control of Tongmai particles.