Simultaneous Determination of 4 Effective Components in Fufang Yuxingcao Tablets by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of chlorogenic acid, forsythoside A, quercitroside and baicalin in Fufang Yuxingcao tablets. METHODS The samples were separated on a CAPCELL PAK MG (4.6 mm×250 mm, 5 μm) column, by a gradient elution using acetonitrile(A) and 0.1% phosphoric acid(B) (0-12 min, 9%A; 12-15 min, 9%→15%A; 15-32 min, 15%A; 32-48 min, 15%→29%A; 48-60 min, 29%A) as mobile phase at a flow rate of 1.0 mL·min^-1. The column temperature was set at 35℃. The wavelength was set on 330 nm. RESULTS The linear ranges of chlorogenic acid, quercitroside, forsythoside A and baicalin were 2-100 μg·mL^-1, 2-100 μg·mL^-1, 4-200 μg·mL^-1 and 12-600 μg·mL^-1(r=0.999 9), the average recoveries(n=6) were 98.0%, 99.8%, 99.1% and 98.3%, respectively, and RSDs were < 2.0%. CONCLUSION A rapid, simple, accurate HPLC method is successfully established for simultaneous determine 4 effective components in Fufang Yuxingcao tablets. The method herein described is helpful for the quality control of Fufang Yuxingcao tablets.