Determination of the 1-(3',4'-Dihydroxycinnamoyl)-cyclopentane-2,3-diol in Angelicae Sinensis Radix by HPLC

OBJECTIVE To establish an HPLC method for determination of 1-(3',4'-dihydroxycinnamoyl)-cyclopentane -2,3-diol in Angelicae Sinensis Radix. METHODS Inertsil ODS-3 C₁₈ column (4.6 mm×250 mm, 5 μm) was adopted with a mobile phase of acetonitrile-0.05% trifluoroacetic acid at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 325 nm and the column temperature was 30℃. RESULTS The good linearity with peak areas was achieved in the rang of 0.023 36-1.168 0 μg (r=1.000 0) for 1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol. The average recovery was 95.33%, and the RSD was 1.88%. CONCLUSION The method is simple, accurate and reproducible, and can be used for the quality control of Angelicae Sinensis Radix.