HPLC测定卡波姆共聚物中残留的丙烯酸含量

Determination of Acrylic Acid in Carbomer Copolymer by HPLC

摘要: 目的采用HPLC测定卡波姆共聚物中残留的丙烯酸含量。方法使用Sepax GP-C₁₈柱(4.6 mm×250 mm，5 μm)分离，流动相为磷酸二氢钾-甲醇(8∶2)，流速为1.0 mL·min^-1，检测波长200 nm，柱温为30 ℃。结果卡波姆共聚物中的残留丙烯酸可达到满意的分离，丙烯酸浓度在0.327 5~32.75 μg·mL^-1内有良好的线性关系(r=0.999 6)，方法平均回收率为93.02%。结论本方法准确，可靠，重现性好，可作为卡波姆共聚物中丙烯酸残留的控制方法。

Abstract: OBJECTIVE To establish an HPLC method for the determination of acrylic acid in carbomer copolymer. METHODS The samples were separated on Sepax GP-C₁₈ column (4.6 mm×250 mm, 5 μm) eluted with potassium dihydrogen phosphate-methanol(80∶20) as mobile phase. The isocratic elution was used at a flow rate of 1.0 mL·min^-1, the detection wavelength was 200 nm, the column temperature was 30 ℃. RESULTS The calibration curve was linear in the range of 0.327 5-32.75 μg·mL^-1 for acrylic acid with r=0.999 6, the average recovery was 93.02%. CONCLUSION The method is accurate and reliable, and can be applied for the quality control of acrylic acid in carbomer copolymer.