Abstract: OBJECTIVE To explore the protective effect of She national medicine (root of Gardenia jasminoides extraction) on bacillus calmette-guérin(BCG) and lipopolysaccharide(LPS)-induced immunological liver injury in mice. METHODS ICR male mice were randomly divided into normal group, model group, the water extract of high, medium and low dose group and bifendate group. Immunological liver injury model were established by intravenously injection of BCG and LPS. The mice in normal group were injected of normal saline. The activity of ALT and AST activity in serum of mice were detected by full automatic biochemical analyzer. ELISA method was used to measure the content of serum IL-2, IL-4, IL-10, TNF-γ. HE staining was used to observe the pathological changes of the liver in mice. RESULTS Compared with the model group, the aqueous extract significantly reduced the activity of ALT and AST(P<0.05); significantly increased the level of IL-2 and IL-10 (P<0.05); and reduced the level of IL-4 and TNF-γ(P<0.05). Compared with the model group, the inflammatory cells in the aqueous extract groups mice liver tissue infiltration, edema, necrosis and immune complex deposit structure were obviously alleviated. CONCLUSION The aqueous extract of She medicine-root of Gardenia jasminoides has protective effect on immune hepatic injury induced by BCG combined with LPS.