Abstract: OBJECTIVE To investigate the protective effect of Tongxinluo capsule on focal cerebral ischemia-reperfusion injury in rats. METHODS Sixty SD rats were randomly divided into the sham-operative group, ischemia-reperfusion group, Tongxinluo capsule low-dose group(0.3 g·kg^-1), medium-dose group(0.6 g·kg^-1) and high-dose group(1.2 g·kg^-1), respectively (n=12). The ischemia reperfusion model rats were induced using suture-occluded method, 30 min of ischemia-reperfusion. after cerebral ischemia 30 min and reperfusion 24 h, to evaluate the score of neurological behavior, using the TTC method measuring the cerebral infarction volume, HE staining to observe the pathological changes of brain tissue and immunohistochemical method to observe the expression of Fas protein. Results Compared with ischemia-reperfusion group, Tongxinluo low, medium and high dose group could significantly improve the symptoms of nervous system damage in rats, reduce cerebral infarcts, Fas protein expression were obviously down-regulated(P<0.01). And compared with Tongxinluo low-dose group, all of them were significantly different in Tongxinluo high-dose group(P<0.05). Conclusion Tongxinluo capsule can inhibit the expression of Fas protein, alleviate nerve function damage after cerebral ischemia, so as to protect the brain tissue.