Abstract: OBJECTIVE To prepare zoledronic acid cationic liposome, and evaluate its characteristics in vitro. METHODS Film dispersion method was used to prepare the zoledronic acid cationic liposome, then the single factor test of formulation and technology was investigated including the evaluation indexes as follows, the entrapment efficiency, drug loading amount, average particle size and Zeta potential. Moreover, its in vitro characterization was studied. RESULTS The prescription process was determined first, in detail, the ratio of DPPC and DC-Chol was 3∶1, PBS hydrated volume was 10 mL, rotary evaporation time was 60 min, ultrasonic averaging time was 5 min, the average particle size, PDI, entrapment efficiency, drug loading and Zeta potential of cationic liposomes were (106.76±1.94 )nm, 0.262±0.027, (38.54±0.99)%, (3.42±0.27)%, +(42.37±2.60)mV, respectively. The profiles of release in vitro was expressed well by Weibull equationin. CONCLUSION The zoledronic acid cationic liposome which prepared by the film dispersion method has relatively high stability, this characteristics would be its basis for pharmacokinetic and pharmacodynamic research in future.