Abstract: OBJECTIVE To establish an HPLC method for determining the contents of the related substances of cinacalcet hydrochloride. METHODS The condition of detection were: Waters C₁₈ column (250 mm×4.6 mm, 5μm), the gradient mobile phase consisted of acetonitrile and 10 mmol·L^-1 potassium dihydrogen phosphate (adjust with 2 mol·L^-1 potassium hydroxide to pH 6.5) with a flow rate of 1.0 mL·min^-1, the UV detection wavelength was 210 nm. RESULTS The resolution of cinacalcet hydrochloride and relate substances was good. The calibration curves were linear in the range of 0.228 6-4.572 μg·mL^-1 for Imp1(r=1.000); the calibration curves were linear in the range of 0.239 6-4.791 μg·mL^-1 for Imp2(r=1.000); the calibration curves were linear in the range of 0.274 1-5.481 μg·mL^-1 for Imp3(r=1.000); the calibration curves were linear in the range of 0.102 9-2.059 μg·mL^-1 for Imp4(r=0.999 8). The average recoveries of Imp1, Imp2, Imp3 and Imp4 were 99.3%, 98.9%, 99.0% and 100.4% respectively; the RSD were 1.8%, 2.9%, 1.7% and 1.7% respectively. CONCLUSION The method is simple, accurate, reliable and can be used for the quality control of the related substances of cinacalcet hydrochloride.