Abstract: OBJECTIVE To study cisplatin and adriamycin on human serum albumin(HSA) interactions. METHODS The interaction of cisplatin and adriamycin on HSA was studied by fluorescence spectra. RESULTS The results showed that cisplatin and adriamycin strongly quenched the fluorescence of human serum albumin. The quenching mevhanisn of cisplatin was a dynamic quenching procedure, and the quenching mevhanisn of adriamycin was a static quenching procedure. CONCLUSION At 17 ℃ and 37 ℃, the binding constant of adriamycin and HSA system are 2.13×104 and 2.76×104 L·mol^-1, furthermore, the binding is 1. When different concentrations of cisplatin is add to the adriamycin and HSA system, the binding constant changed, and the binding site is still 1.