UPLC同时测定复方守宫散中核苷类含量

Simultaneous Determination of Nucleoside Contents in Compound Shougong Powder by UPLC

摘要: 目的建立UPLC同时测定复方守宫散中腺嘌呤、腺苷含量的方法。方法色谱柱Acquity BEH C₁₈(2.1 mm× 100 mm，1.7 μm)，甲醇-水为流动相，梯度洗脱，流速：0.25 mL·min^-1，柱温：26 ℃，检测波长：254 nm。结果腺嘌呤、腺苷的进样量分别在0.040 9~0.511 5 mg·mL^-1(r=0.999 9)和0.057 8~0.722 0 mg·mL^-1(r=0.999 6)内与其峰面积呈良好的线性关系；平均回收率分别为97.7%，97.7%，RSD分别为0.96%，1.18%。结论该方法快速、可靠、准确，可作为复方守宫散的质量控制方法。

Abstract: OBJECTIVE To establish a UPLC method for the determination of adenine and adenosine in Compound Shougong powder. METHODS The determination was performed on Acquity BEH C₁₈(2.1 mm×100 mm, 1.7 μm) with mobile phase consisted of methabol-water(gradient elution) at a flow rate of 0.25 mL·min^-1. Column temperature was 26 ℃. The UV detection wavelength was set at 254 nm. RESULTS The calibration curves were linear in the range of 0.040 9-0.511 5 mg·mL^-1(r=0.999 9) for adenine, 0.057 8-0.722 0 mg·mL^-1(r=0.999 6) for adenosine. The average recoveries were 97.7%, 97.7% and RSDs were 0.96%, 1.18%, respectively. CONCLUSION The determination method is fast, reliable, accurate, and can be used for quality control of Compound Shougong powder.