HPLC测定保健食品中微胶囊型维生素A

Determination of Vitamin A in the Form of Microcapsule in Functional Food by HPLC

摘要: 目的建立测定保健食品中微胶囊型维生素A含量的反相高效液相色谱法。方法以适宜浓度氨水溶液破坏保健食品中微胶囊型维生素A的微胶囊结构，再以有机溶剂提取。采用Agilent ZORBAX SB-C₁₈色谱柱(250 mm×4.6 mm，5 μm)，以甲醇为流动相，流速1.0 mL·min^-1，检测波长为325 nm，柱温35 ℃。结果维生素A在1.41~56.4 μg·mL^-1内线性关系良好(r=1.000)；平均回收率为97.5%(RSD=1.9%)。结论本法方法简单、准确，重现性好，适用于保健食品中微胶囊型维生素A的测定。

Abstract: OBJECTIVE To develop an HPLC method for determination of vitamin A in the form of microcapsule in functional food. METHODS Microcapsule structure was disrupted by suitable ammonia solution and vitamin A was extracted by organic solutions. The chromatographic separation was achieved on an Agilent ZORBAX SB-C₁₈ column(250 mm×4.6 mm, 5 μm) with methanol as the mobile phase. The detection wavelength was at 325 nm, flow rate 1.0 mL·min^-1 and the column temperature 35 ℃. RESULTS The linear range of vitamin A was 1.41-56.4 μg·mL^-1(r=1.000). The average recovery of vitamin A was 97.5%(RSD=1.9%). CONCLUSION The established method was simple, accurate and with good reproducibility, suitable for content determination of vitamin A in functional food.