Abstract: OBJECTIVE To establish the chromatographic fingerprint of Jingzhi Guanxin Keli Fufang by HPLC. METHODS HPLC was performed on Jingzhi Guanxin Keli Fufang using Agilent 1100 DAD-HPLC. Inertsil ODS-3 column (4.6 mm×250 mm, 5 μm) was used; the mobile phase was acetonitrile-0.1% phosphoric acid for gradient elution; the flow rate was 1 mL·min^-1; the detection wavelength was 270 nm; the column temperature was 30 ℃, and inject volume was 10 μL. RESULTS Twenty-two HPLC common peaks were acquired in the 10 batches of four kinds of drugs of Jingzhi Guanxin Keli Fufang, and their similarities were >0.90. Good precision, stability and repetition were showed. CONCLUSION A reliable method is provided for the quality identification of Jingzhi Guanxin Keli Fufang. And the chemical components of different germplasms of Jingzhi Guanxin Keli Fufang are different from each other; selection of germplasm is important in quality control of Jingzhi Guanxin Keli Fufang.