Abstract: OBJECTIVE To study the effect of Ganshu capsule(GSC) on treating the liver fibrosis of rats induced by dimethylnitrosamines(DMN). METHODS The animal model of liver fibrosis in rats was induced with DMN. The liver and spleen index, liver tissue morphology and hydroxyproline content were observed. And radioimmunoassay was used to detect serum hyaluronic acid(HA), laminin protein(LN), Ⅳ collagen(Ⅳ-C) and pre-collagen type Ⅲ(PCⅢ). RESULTS The high and middle dose group of GSC could not only reduce liver index, spleen index, liver hydroxyproline content significantly, but also reduce serum HA, LN, IV-C and PCIII level significantly in rats(P<0.01). And the high dose group of GSC reduced the liver index significantly better than colchicine group(P<0.01). GSC could also improve liver tissue morphology. CONCLUSION GSC has a good effect on the treatment of liver fibrosis.