Abstract: OBJECTIVE　To study the chemical constituents in Chrysanthemum indicum L. METHODS Extract chemical continents from Chrysanthemum indicum L. by using 80% alcohol and silica gel column chromatography methods. The structure of the isolated compounds were identified by physicochemical identification and spectral analysis methods, such as UV, FTIR, EI-MS, ESI-MS, 1H-NMR, 13C-NMR, and so on. RESULTS Eleven compounds were isolated from the ethyl acetate extracts, chloroform extracts and the n-butanol extracts of Chrysanthemum indicum L. They were chrysoeriol(Ⅰ) , luteolin(Ⅱ) , luteolin-7-O-β-D-glucopyranoside (Ⅲ), apigenin(Ⅳ), β-sitosterol(Ⅴ), kaempferol(Ⅵ), chrysophanol(Ⅶ), linarin(Ⅷ), acacetin(Ⅸ), acacetin 7-O-(6″-O-acetyl)-β-D-glucopyranoside(Ⅹ) and acacetin-7-O-β-D-rutinoside(Ⅺ), respectively. CONCLUSION Among them, compounds Ⅹ and Ⅺ were isolated from Chrysanthemum indicum L. for the first time.