HPLC-ELSD测定复方益母草胶囊中盐酸水苏碱的含量

Studies on the Determination of Stachydrine Hydrochloride in Compound Motherwort Capsules by HPLC-ELSD

摘要: 目的建立复方益母草胶囊中盐酸水苏碱的HPLC含量测定方法。方法高效液相色谱法，采用Agilent ZORBAX NH₂(4.6 mm×250 mm，5 mm)色谱柱，流动相为乙腈-水(83∶17)，流速为1.0 mL·min^-1，采用蒸发光散射检测器检测，对数法计算含量。结果盐酸水苏碱对照品线性范围为5.32~15.96 mg，r=0.999；平均回收率为98.66%，RSD为1.9%(n=9)。结论本方法快速、简便、重复性好、专属性强且准确度高。

Abstract: OBJECTIVE To establish an HPLC method to determine the content of Stachydrine hydrochloride in compound motherwort capsules. METHODS The Agilent ZORBAX NH₂ column was used. The mobile phase was acetonitrile and water (83∶17). The flow rate was 1.0 mL·min^-1. The detector was ELSD. Logarithmic method was used to calculate the content. RESULTS The linear range of reference substance of Stachydrine hydrochloride was 5.32~15.96 μg(r=0.999); The average recovery rate was 98.66% and the relative standard deviation(RSD) was 1.9%(n=9). CONCLUSION The quantitaive method is rapid, simple, highly specific with good repeatability and accuracy.