Abstract: OBJECTIVE To analyse Polygoni Cuspidati Rhizoma et Radix from different areas by HPLC-DAD, and to establish the method for identification and quality control of it. METHODS YMC-C₁₈ column (4.6 mm×250 mm, 5 μm) was used. Analysis was performed on condition of gradient elution solvents of acetonitrile and 0.2% phosphoric acid. The flow rate was 1.0 mL·min^-1, detecting wavelength was 280 nm and column temperature was 25 ℃. Eight components of five batches of Polygoni Cuspidati Rhizoma et Radix were determined, and the fingerprint chromatogram of it was established. RESULTS The method of content determination was desirable precision, reproducibility, and stability. Seventeen common peaks were established from specimens, the similarity of samples was 0.915-0.973. CONCLUSION This method indicates the difference of the chemical component in Polygoni Cuspidati Rhizoma et Radix from various habitats in China, and can be used for quality control.