穿膜肽提高纳米银穿膜活性的研究

The Penetrating Activity of Nano-silver Mediated by Cell Penetrating Peptide

摘要: 目的合成纳米银，在其表面修饰穿膜肽(TAT)，并检测修饰后纳米银粒对人乳腺癌耐阿霉素细胞(MCF-7/ADR)的穿膜活性。方法通过化学还原法制备纳米银(AgNP)，并通过Ag-S共价键与TAT连接修饰AgNP。通过粒度仪、透射电镜、激光共聚焦显微镜、流式细胞仪以及二喹林甲酸法等仪器及方法对其表征、共价连接及TAT的介导活性进行测定。结果成功制备了10 nm以下的AgNP，且修饰TAT后的AgNP(AgNP-TAT)表现出了比AgNP 更强的穿膜活性。结论 TAT修饰AgNP后能显著提高其穿膜活性。

Abstract: OBJECTIVE To assess the intracellular delivery efficiency of silver nanoparticles surface-modified by cell penetrating peptides(TAT) in human breast cancer drug doxorubicin-resistant MCF-7/ADR cells. METHODS Silver nanoparticle(AgNP) was prepared by chemical reduction and was modified with TAT by Ag-S covalent bond. The TAT-AgNP was characterized using particle analyzer, TEM, laser confocal microsopy, and TGA, and TAT-mediated cellular uptake were measured using FACS. RESULTS The AgNP with diameter below 10 nm was successfully synthesized. The cell penetrating activity of TAT-modified AgNP (AgNP-TAT) was higher than that of AgNP. CONCLUSION The cell penetrating activity of AgNP was significantly improved by modification with TAT.