高级检索

    大黄5个蒽醌类成分与大鼠血浆蛋白结合率的测定

    Determination of the Binding Rate of Rat Plasma Protein with Five Anthraquinones in Rhubarb

      摘要
    • 摘要: 目的 建立测定大黄苷元中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚5种成分与大鼠血浆蛋白结合率的方法。方法 采用体外平衡透析法，模拟大黄苷元与血浆蛋白的结合过程，以固相萃取柱处理血浆样品，用高效液相色谱法测定大黄苷元中5种蒽醌类成分在透析袋内血浆中的浓度与透析袋外缓冲液中的浓度，计算血浆蛋白结合率。结果 芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚提取回收率分别为68.6%，91.5%，78.6%，88.2%和71.4%，与大鼠体外血浆蛋白结合率分别为93.1%，91.3%，95.2%，87.5%和89.7%。结论 大黄5种苷元与大鼠血浆蛋白结合率较高，且蛋白结合率与血药浓度无明显依赖性。

       

      Abstract: OBJECTIVE To develop a method for the simultaneous determination of the binding rate of plasma protein with aloe-emodin, rhein, emodin, chrysophanol and physcion. METHODS The equilibrium dialysis method was carried out to imitate the binding process between five anthraquinones and plasma protein. Plasma samples were treated with solid-phase extraction and the binding rate was determined by HPLC. RESULTS The extract recoveries of aloe-emodin, rhein, emodin, chrysophanol and physcion were 68.6%, 91.5%, 78.6%, 88.2% and 71.4%, the binding rates of plasma protein were 93.1%, 91.3%, 95.2%, 87.5% and 89.7%. CONCLUSION This method shows the high binding rate of five anthraquinones to plasma protein. The binding rates of five anthraquinones are not proportionally dependent on plasma concentration.

       

      • HTML全文
      • 参考文献(1)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回