FGF21对HepG2细胞TGF-β信号通路的影响

Effect of FGF21 in TGF-β Signaling Pathway of HepG2 Cells

摘要: 目的研究FGF21对TGF-b/Smads信号通路中相关蛋白和mRNA表达的影响。方法采用Western blot法检测HepG2 cells的TGF-b1，TGF-b RⅡ，Smad 2，3，4，7的蛋白表达水平和采用Q-PCR方法检测HepG2 cells的TGF-b1，TGF-b RⅡ，Smad 2，3，4，7的mRNA表达水平。结果在不同FGF21浓度下，TGF-b1，TGF-b RⅡ，Smad 2，3，4，7蛋白和mRNA表达水平不同(P<0.05)。结论 FGF21通过促进TGF-β信号通路中的信号分子或受体的表达，或者通过下调此信号通路的阻断分子而抑制肿瘤发生。

Abstract: OBJECTIVE To study the effects of FGF21 on relative proteins and mRNA expression involved in TGF-b/Smads signaling pathway. METHODS Detect the protein expressions of TGF-β1, TGF-β RⅡ, Smad 2,3,4,7 of HepG2 cells with Western blot and detect the mRNA expression of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 of HepG2 cells with Q-PCR. RESULTS The expressions of the protein and mRNA of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 varied with the different concentrations of FGF21(P<0.05). CONCLUSION FGF21 might inhibit tumor by promoting TGF-β signaling pathway in the expression of signaling molecules or receptors, or by decreasing the blocking molecules of this signaling pathway.