HPLC测定翅茎白粉藤中白黎芦醇的含量

Determination of Resveratrol in Cissus Hexangularis by HPLC

摘要: 目的建立翅茎白粉藤中白黎芦醇的含量测定方法。方法采用高效液相色谱法，色谱柱XB-C18(4.6 mm×250 mm，5 μm)，以乙腈-水(25∶75)为流动相，检测波长为306 nm，柱温为室温。结果白黎芦醇在2.50~40.0 μg内峰面积与其浓度呈良好线性关系(r=0.999 9)，平均回收率为97.3%，RSD为0.98%(n=6)。结论本方法简便、准确、重复性好，可作为该药材含量测定方法。

Abstract: OBJECTIVE To develop an HPLC method for detecting resveratrol in Cissus hexangularis. METHODS An XB-C18 column(4.6 mm×250 mm, 5 mm) was used. The mobile phase consisted of acetonitrile-water(25∶75). The detection wavelength was 306 nm and the column temperature was room temperature. RESULTS Resveratrol showed a good linear relationship in the range of 2.50-40.0 μg·mL-1, r=0.999 9. The recovery and RSD were 97.5% and 0.98%(n=6), respectively. CONCLUSION This method is simple, accurate and repeatable to determine the content of resveratrol in Cissus hexangularis.