均匀试验设计优选青黛提取工艺

Optimal Extraction Technology of Indigo Naturalis by Uniform Design

摘要: 目的通过均匀试验优选青黛中靛玉红的最佳提取工艺。方法以靛玉红提取率为指标，对青黛醇提过程中加醇量、乙醇浓度、提取时间及提取次数4个因素进行筛选。结果影响靛玉红提取率的各因素作用大小顺序为：乙醇浓度＞溶剂量＞提取时间＞提取次数，其中乙醇浓度，提取次数，溶剂量与靛玉红提取率呈正相关。结论青黛最佳提取工艺为加90%乙醇9倍量，提取2次，每次0.5 h。

Abstract: OBJECTIVE To optimize extraction process of Indigo Naturalis by uniform design. METHODS The process was studied by uniform design method with the extraction ratio of indirubin as the index, and four factors were chosen, including ethanol volume, ethanol concentration, extraction time and the extraction times. RESULTS The factors influenced the indirubin extraction was ethanol concentration＞ethanol volume＞extraction time＞extraction times. Extraction time, ethanol volume and ethanol concentration were positively correlated with the extraction efficiency. CONCLUSION The results indicate that the optimum extraction technology is as follows: 10 times volume of 90% ethanol, 0.5 h reflux and extraction for 2 times.