Abstract: OBJECTIVE To study toosendanin extraction methods in Fructus toosendan. METHODS The toosendanin was extracted separately by the water bath extraction, soxhlet extraction and ultrasonic cell crushing extraction. The content of toosendanin was determinated by HPLC. RESULTS Using orthogonal experiments analysis, the optimal extraction conditions of these three extraction methods were as follows: ①The water bath extraction method: 50 ℃, 80% ethanol, extraction time 2 h, ratio of material to solvent 1∶30; ②The soxhlet extraction method: 85 ℃, 70% ethanol, extraction time 7 h, ratio of material to solvent 1∶20; ③The ultrasonic cell crushing extraction method: 250 W, 70% ethanol, extraction time 45 min, ratio of material to solvent 1∶30. CONCLUSION The established methods are simple, reliable and reproducible, which could provide some data basis in a comprehensive study of Fructus toosendan.