HPLC测定铁皮石斛饮料中人参皂苷Rb1的含量

Determination of the Content of Ginsenoside Rb1 in Tiepishihu Beverage by HPLC

摘要: 目的建立测定铁皮石斛饮料中人参皂苷Rb1含量的方法。方法采用Agilent Eclipse XDB-C₁₈(4.6 mm×250 mm，5 µm)色谱柱；流动相：乙腈-0.1%磷酸溶液(32∶68)；流速：1.0 mL·min^-1；进样体积：10 µL，柱温：40 ℃；检测波长：203 nm。结果人参皂苷Rb1在1.012~5.06 µg内呈良好的线性关系(r=1.000 0)，平均加样回收率为102.3%，RSD为1.8%(n=6)。结论该法准确，简便，快速，灵敏度高，重复性好，适用于铁皮石斛饮料中人参皂苷Rb1的含量测定。

Abstract: OBJECTIVE To establish an HPLC method for determination of the content of ginsenoside Rb1 in Tiepishihu beverage. METHODS An Agilent Eclipse XDB-C₁₈ column(4.6 mm×250 mm, 5 µm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(32∶68), the flow rate was 1.0 mL·min^-1, the wavelength of detector was 203 nm, the injection volume was 10 µL and column temperature was set at 40 ℃. RESULTS The calibration curves showed good linearity in the range of 1.012-5.06 μg. The average recovery(n=6) was 102.3%, RSD was 1.8%. CONCLUSION The method was simple, fast and accurate for analysis of ginsenoside Rb1 in Tiepishihu beverage.