Abstract: OBJECTIVE To establish an HPLC method for simultaneously determining the alkaloids of Kexieling tablets in rat plasma. METHODS The plasma samples were determined at 220 nm wavelength by reversed-phase HPLC and ultraviolet detection following oral administration of Kexieling tablets. RESULTS Sophoridine and matrine with 350 and 100 ng·mL-1of limits of quantitation in plasma, respectively, can be accurately quantitated, and the recovery of both analytes was greater than 98%.CONCLUSION The method is simple,accurate and can be referenced for pharmacokinetic study of Kexieling tablets.