包载荧光探针香豆素-6的PLGA纳米粒的制备

Preparation of Fluorescent Probe Coumarin-6 Loaded PLGA Nanoparticles

摘要: 目的以生物可降解材料聚乳酸-羟基乙酸共聚物(PLGA)为材料，制备包载荧光探针香豆素-6 纳米粒，并进行处方优化。方法采用乳化-溶剂挥发法制备香豆素-6-PLGA纳米粒，以利用率和包封率为考察指标，正交试验对处方进行优化。结果制得香豆素-6纳米粒的包封率为51.6%，利用率为81.9%，平均粒径135 nm，香豆素-6体外72 h泄漏率低于2%。结论香豆素-6 PLGA纳米粒制备工艺简便可行，香豆素-6可作为荧光探针，用于纳米粒的动物体内示踪及靶向性研究。

Abstract: OBJECTIVE To prepare fluorescent probe coumarin-6 loaded nanoparticles using the biodegradable material poly(lactic-co-glycolic acid)(PLGA) as carrier. METHODS Coumarin-6 loaded PLGA nanoparticles were prepared by emulsification solvent evaporation method. The formulation was optimized by orthogonal design using utilization ratio and entrapment efficiency as the indexes. RESULTS The mean entrapment efficiency of coumarin-6 loaded PLGA nanoparticles was 51.6% and the utilization ratio was 81.9%. The average size was 135 nm and the in vitro cumulative release of coumarin-6 from nanoparticles was less than 2% in 72 h. CONCLUSION The preparation process of coumarin-6 loaded PLGA nanoparticles is simple and feasible, and coumarin-6 can be used as fluorescence marker for in vivo tracing and targeting study of nanoparticles.