莱菔子脂溶性成分的GC-MS分析

Analysis of Fat-soluble Components in Raphani Semen by GC-MS

摘要: 目的探讨莱菔子脂溶性成分的组成及相对含量。方法采用超声法提取莱菔子油，KOH甲醇溶液甲酯化，通过气相色谱-质谱联用技术分析莱菔子中脂溶性成分。结果从莱菔子油中共分离鉴定出27中脂溶性成分，15种脂肪酸成分，其相对含量占总成分的50.47%；另有12种成分未见文献报道。结论本方法简便、快速、分离效果好，适用于莱菔子脂溶性成分的分析研究。

Abstract: OBJECTIVE To discuss the constituents and reletive contents on fat-soluble components in Raphani Semen. METHODS The oil from Raphani Semen was extracted by ultrasonic technology, then esterified with KOH-CH3OH and analysised by GC-MS. RESULTS There were 27 fat-soluble components identified, inclouding 15 fatty acids components which were accounting for 50.47% of the total components, 12 components had not been reported in documents. CONCLUSION The method is simple, efficient and could be used for the study of the fat-soluble components in Raphani Semen.