Abstract:
OBJECTIVE To fully utilize the local medicinal resource Nymphaea candida C.Presl from Xinjiang, this study employs ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS) to identify the chemical constituents of Nymphaea candida C.Presl.
METHODS A Thermo Hypersil GOLD aQ chromatographic column(2.1×150 mm, 1.9 μm) was used, with 0.1% aqueous formic acid solution-methanol as the mobile phase for gradient elution. The flow rate was 0.3 mL·min−1, the column temperature was 35 ℃, and electrospray ionization (ESI) source was applied with scanning under both positive and negative ion modes.
RESULTS By comparison with reference standards, accurate molecular masses from MS1, and fragment information from MS2, a total of 72 chemical constituents were identified from Nymphaea candida C.Presl, including 27 organic acids, 20 flavonoids, 7 hydrolyzable tannins, 4 phenylpropanoids, 2 coumarins, and 2 terpenoids. Active ingredients such as corilagin, brevifolin carboxylic acid and gentiopicrin were also detected in Nymphaea candida C.Presl.
CONCLUSION This study systematically elucidates the main chemical constituents of Nymphaea candida C.Presl using UHPLC-Q-TOF-MS technology. The identification results demonstrate that the flowers of Nymphaea candida C.Presl contain a variety of bioactive chemical constituents, which provides a research basis for its subsequent efficacy research and the development of corresponding functional products.
下载: