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    基于UPLC-QqQ-MS/MS法联合多元统计分析及熵权TOPSIS法评价白术饮片质量

    Based on UPLC-QqQ-MS/MS Combined with Multivariate Statistical Analysis and Weighted TOPSIS Method to Evaluate the Quality of Atractylodes Macrocephala Koidz.

      摘要
    • 摘要:
      目的  对白术饮片的质量进行系统的评价，从而保证白术饮片的质量和临床疗效。
      方法 采用超高效液相色谱-三重四极杆串联质谱(UPLC-QqQ-MS/MS)技术对白术饮片中白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ、异绿原酸A、异绿原酸B、异绿原酸C、隐绿原酸、绿原酸、新绿原酸和1,3-二咖啡酰奎宁酸的含量进行测定；采用多元统计分析方法考察白术饮片的产地、生长年限、加工和炮制方法对外观性状及有效成分含量的影响；利用熵权TOPSIS法构建白术药材质量评价模型，并考察不同影响因素对白术饮片质量的影响。
      结果 本研究所建立的白术中活性成分的含量测定方法稳定、可行；通过对不同白术饮片质量进行分析发现，产地对白术饮片中倍半萜内酯类和酚酸类成分含量具有显著影响，浙江产白术的倍半萜内酯类和酚酸类化合物的含量较高；生长年限对白术饮片中倍半萜内酯类和酚酸类成分含量也具有显著影响，多年生白术饮片中倍半萜内酯类和酚酸类成分含量更高；切制途径和干燥方法对白术饮片中倍半萜内酯类和酚酸类成分含量也具有显著影响；麸炒却可令不同因素影响下的白术饮片所含化学成分趋同。最后，通过熵权TOPSIS分析也证实，浙江产地、生长≥2年、软化切制后经过烘干方式得到白术饮片质量最佳。
      结论 本研究所建立的研究方法可用于白术饮片的质量评价。

       

      Abstract:
      OBJECTIVE  In order to ensure the quality and clinical efficacy of Atractylodes macrocephala Koidz. pieces, a systematically evaluation was conducted.
      METHODS  The contents of atractylenolide Ⅰ, atractylenolide Ⅱ, atractylenolide Ⅲ, isochlorogenic acid A, 1,3-dicaffeoylquinic acid, isochlorogenic acid C, isochlorogenic acid B, cryptochlorogenic acid, chlorogenic acid and neochlorogenic acid in Atractylodes macrocephala Koidz. were determined by ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QqQ-MS/MS). Multivariate statistical analysis was used to investigate the effects of origin, growing years and processing methods on the appearance and the contents of active ingredient in Atractylodes macrocephala Koidz.. The quality evaluation model of Atractylodes macrocephala Koidz. was constructed by the method of entropy weight TOPSIS. Then the effects of different influencing factors on the quality of Atractylodes macrocephala Koidz. pieces were investigated.
      RESULTS  The content determination method of active ingredients in Atractylodes macrocephala Koidz. was established in this study which was stable and feasible. Through the analysis of the content of active ingredients in Atractylodes macrocephala Koidz., it was found that the origin had a significant effect on sesquiterpene lactones and phenolic acids. It was higher in the origin of Zhejiang. The growth years also had a significant effect on the content of sesquiterpene lactones and phenolic acids. It was higher in the perennial Atractylodes macrocephala Koidz. pieces. Cutting and drying method also had significant effect on the content of sesquiterpene lactones and phenolic acids. Although the content of active ingredients differed among various sources of Atractylodes macrocephala Koidz., bran stir-frying could converge their levels. Finally, the entropy weight TOPSIS analysis also confirmed that the quality of Atractylodes macrocephala Koidz. pieces which was higher in origin of Zhejiang, perennial, processing by softening cutting and oven drying.
      CONCLUSION  The research method was established in this study, which could be used to evaluate the quality of Atractylodes macrocephala Koidz..

       

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