不同基原羌活标准汤剂的指纹图谱建立及其化学计量学分析

吕渭升; 位翠杰; 卢晓莹; 何广铭; 罗睿; 李振雨; 贾小舟; 陈向东; 孙冬梅

doi:10.13748/j.cnki.issn1007-7693.20240531

不同基原羌活标准汤剂的指纹图谱建立及其化学计量学分析

Fingerprint Establishment of Notopterygii Rhizoma et Radix Standard Decoction from Different Origins and Their Chemometrics Analysis

摘要

摘要:
目的建立不同基原羌活标准汤剂UHPLC指纹图谱分析方法，为其质量评价提供依据。
方法通过UHPLC构建26批羌活标准汤剂和宽叶羌活标准汤剂的指纹图谱，采用中药色谱指纹图谱相似度评价系统(2012A版)对指纹图谱进行相似度评价，并进行聚类分析、主成分分析及正交偏最小二乘-判别分析。
结果选取了14个共有峰，指认其中11个成分；羌活、宽叶羌活标准汤剂的相似度分别为0.939~0.988、0.943~0.989；化学计量学结果显示26批样品分为2类，并筛选出紫花前胡苷、6′-O-(反式阿魏酰基)-紫花前胡苷、阿魏酸、异绿原酸B、绿原酸5个对组分差异影响较大的成分，能够反映26批次不同基原羌活标准汤剂特征性质量差异。
结论建立的方法可快速区分和评价不同基原羌活标准汤剂和鉴别其相关伪品，为羌活相关中药制剂质量控制提供参考。

Abstract:
OBJECTIVE To establish a UHPLC fingerprint analysis method for different origins of standard decoction of Notopterygii Rhizoma et Radix(NRR), so as to provide a theoretical basis for its quality evaluation.
METHODS The fingerprints of 26 batches of Notopterygium incisum Ting ex H. T. Chang and Notopterygium franchetii H. de Boiss. standard decoction samples were established by UHPLC, and the similarity evaluation of UHPLC fingerprints of standard decoction of NRR was carried out using the traditional Chinese medicine chromatographic fingerprint similarity evaluation system(2012A version), and cluster analysis, principal component analysis and orthogonal partial least squares-discriminant analysis were performed.
RESULTS Fourteen chromatographic peaks were selected as common peaks, and 11 components were identified. The similarity of standard decoction samples of Notopterygium incisum Ting ex H. T. Chang and Notopterygium franchetii H. de Boiss. were 0.939-0.988 and 0.943-0.989, respectively. The results of chemometrics showed that the 26 batches of NRR standard decoction samples were clustered into 2 types and 5 components with large differences in composition were screened out including nodakenin, 6′-O-trans-feruloylnodakenin, ferulic acid, isochlorogenic acid B and chlorogenic acid.
CONCLUSION The established methods can quickly identify and evaluate the origins of standard decoction of NRR and counterfeits, it provides reference for the quality control of Chinese medicinal preparation related to NRR.

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