基于UPLC-Q-Exactive-Orbitrap MS/MS的白豆蔻水提物化学成分及入血成分分析

巴达拉胡; 吴杰斯; 包白音木其尔; 刘小伟; 敖道夫

doi:10.13748/j.cnki.issn1007-7693.20233045

基于UPLC-Q-Exactive-Orbitrap MS/MS的白豆蔻水提物化学成分及入血成分分析

Analysis of Chemical Constituents and Components Absorbed into Blood of Baidoukou Aqueous Extract Based on UPLC-Q-Exactive-Orbitrap MS/MS

摘要

摘要:
目的采用UPLC-Q-Exactive-Orbitrap MS/MS技术，对白豆蔻水提物主要化学成分及大鼠口服入血成分进行分析。
方法采用ACQUITY UPLC HSS T3(2.1 mm×100 mm，1.8 μm)色谱柱；以A(去离子水，含0.1%甲酸)和B(乙腈，含0.1%甲酸)为流动相，梯度洗脱，柱温：35 ℃；进样体积：10 μL；流速：0.2 mL·min⁻¹。采用电喷雾离子源(ESI)，Full MS/dd-MS²模式检测。
结果从白豆蔻水提物中共鉴定70种成分，包括有机酸类、黄酮类、氨基酸类、生物碱类、内酯类等。含药血清样本中分析19种原型成分，包括青蒿酸、壬二酸等10种有机酸类，异土木香内酯、去氢木香内酯2种内酯类，甜菜碱、黄嘌呤2种生物碱类，酪氨酸、色氨酸等3种氨基酸。
结论本研究首次阐明了白豆蔻水提物化学成分及其口服入血成分，为其进一步研究与开发提供科学的理论依据。

Abstract:
OBJECTIVE To analyze the main components of Baidoukou aqueous extract and the components absorbed into blood of rats after oral administration by UPLC-Q-Exactive-Orbitrap MS/MS technology.
METHODS Using ACQUITY UPLC HSS T3(2.1 mm×100 mm, 1.8 μm) column; using A(deionized water, containing 0.1% formic acid) and B(acetonitrile, containing 0.1% formic acid) as mobile phases with gradient elution, column temperature was 35°C, while injection volume was 10 μL, and flow rate was 0.2 mL·min⁻¹. Using electrospray ionization source(ESI), detect with Full MS/dd-MS² mode.
RESULTS A total of 70 components were identified from the aqueous extract of Baidoukou, including organic acids, flavonoids, amino acids, alkaloids, and lactones. Nineteen prototype components were identified in the drug-containing serum samples, including 10 organic acids such as artemisinic acid and azelaic acid, 2 lactones such as isoalantolactone and dehydrocostus lactone, 2 alkaloids such as betaine and xanthine, 3 amino acids such as L-tyrosine and L-tryptophan.
CONCLUSION This study sclarified the chemical composition of Baidoukou aqueous extract and its oral blood components for the first time, providing a theoretical basis for its further research and development.

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