Abstract: OBJECTIVE To construct an index system that can reflect the characteristics and development law of pharmacy, so as to promote the connotative development of pharmaceutical higher education in China. METHODS Using the methods of literature analysis and comparative research, this paper analyzes the connotation of pharmacy and the influencing factors of discipline competitiveness. The content and weight of the evaluation index system are determined by two rounds of Delphi method and analytic hierarchy process. RESULTS The evaluation index system of pharmaceutical discipline competitiveness includes 4 first-class indicators: discipline resource competitiveness, discipline output competitiveness, discipline management competitiveness and discipline environment competitiveness, included 11 secondary indicators faculty, discipline platform, discipline reputation, scientific research projects and scientific research funds, talent training, scientific research level, social service characteristics and contributions, discipline culture and system construction, information construction, government funding and market competitiveness. There were 34 tertiary indicators and 70 detailed indicators. CONCLUSION The evaluation index system integrates the elements of discipline management, discipline culture and discipline environment, diversified evaluation participants and evaluation methods, and includes the disclosure of bad academic behavior and the characteristic indicators of pharmacy. Compared with the existing evaluation index system, it can more scientifically and comprehensively evaluate the construction of pharmaceutical discipline in colleges and universities.