Abstract: OBJECTIVE To explore the effect of LncRNA Linc00152 on the proliferation, invasion and migration of gastric cancer cells by miR-193a-3p sponging.METHODS The expression level of Linc00152 in normal gastric mucosal cells(GES-1) and four gastric cancer cells(MGC803, BGC803, SGC7901, AGS) was detected by qRT-PCR. MGC-803 cells were divided into pcDNA3.1-GFP-Linc00152 group, pcDNA3.1-GFP group, while AGS cells were divided into si-Linc00152 group and si-NC group. Each group was treated with corresponding Linc00152 overexpression and knockdown vector, gene levels of Linc00152. Cyclin D1(CCND1) and miR-193a-3p were measured by qRT-PCR, cell proliferative ability was tested by CCK-8 and colony formation assay, apoptosis of transfected cells was detected by flow cytometry after Annexin VFITC/PI staining, invasion ability was observed by Transwell, luciferase reporter assay was performed or measuring the relationship between Linc00152 and miR-193a-3p. Western blotting was used to determine the protein levels of Bcl-2, Bax, CCND1, E-cadherin and Vimentin. The cells of si-Linc00152 and si-NC group were inoculated into nude mice to observe the body weight and general state of nude mice, and the tumor volume and weight were recorded after death. The expression levels of Linc00152, CCND1 and miR-193a-3p in tumor tissue were measured by qRT-PCR.RESULTS The expression of Linc00152 in gastric cancer cells MGC-803, BGC-803, SGC-7901 and AGS was higher than that in GES-1 cells, in which Linc00152 was lower in gastric cancer MGC-803 cells and higher in gastric cancer AGS cells. The MGC-803 cells and AGS cells were used for further experiment. Compared with pcDNA3.1-GFP group, cell proliferative ability, cell invasion and migration ability, and the gene and protein levels of CCND1, protein levels of Bcl-2 and vimentin were increased notably, and apoptosis, the gene level of miR-193a-3p, protein level of E-cadherin were decreased significantly in pcDNA3.1-GFP-Linc00152 group. In the Luciferase reporter assay, miR-193a-3p overexpression resulted in a significant decrease in luciferase activity of the Linc00152-WT reporter, but no effect on Linc00152-MUT luciferase activity. Compared with si-NC group, the volume and weight of tumour, gene levels of CCND1 and Linc00152 in vivo were decreased markedly, and the gene level of miR-193a-3p increased notably in si-Linc00152 group.CONCLUSION Linc00152 targeting miR-193a-3p on the proliferation, invasion and migration of gastric cancer cells, and the mechanism related to cell cycle, epithelial-mesenchymal transition and apoptosis.