Abstract: OBJECTIVE To establish an HPLC fingerprint of Qiju Dihuang pill, and provide an effective and reliable method for the quality evaluation and quality control of Qiju Dihuang pill. METHODS The HPLC fingerprint analysis was carried out on a column of CAPCELL PAK MG C₁₈(250 mm×4.6 mm, 5 μm), using a mobile phase of acetonitrile-0.1% phosphoric acid solution. The detection wavelength was set at 225 nm, and the total run time was 80 min. The control fingerprint was established by similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine, and the similarity of 239 batches of samples was evaluated. RESULTS There were 23 characteristic common peaks in HPLC chromatograms and all the characteristic common peaks were identified by comparison of both the reference substance and the reference herbs. The similarity of the 238 batches in 239 batches of samples was >0.9, and the similarity was good. CONCLUSION The established HPLC fingerprint information of Qiju Dihuang pill can be used for the quality control, and provide a basis for the overall quality evaluation and control of Qiju Dihuang pill.