Abstract: OBJECTIVE To optimize the preparation process of Alpinia officinarum Hance volatile oil wrapped with b-cyclodextrin(b-CD) using the colloid mill method. METHODS With yield of inclusion compound and inclusion rate as evaluation indexes, the single factor test combining Box-Behnken response surface methodology was used to study the effects of the ratio of b-CD to Alpinia officinarum Hance volatile oil, the ratio of water to b-CD, and inclusion time on the inclusion process. Inclusion complexes were verified by IR and microscopic image. RESULTS The optimized parameters of inclusion were as follows:the ratio of b-CD to Alpinia officinarum Hance volatile oil was 10:1, the ratio of water to b-CD was 5:1, and the inclusion time was 45 min. CONCLUSION The inclusion of Alpinia officinarum Hance volatile oil by colloid mill is reasonable and feasible.