Abstract: Transdermal drug delivery system has the advantages of convenient administration, stable blood concentration and no first-pass effect, but the barrier effect of skin makes it difficult for drugs to penetrate the skin. In recent years, a lot of new transdermal drug carriers have emerged, such as liposomes, ethosomes, and vesicles, etc., which can promote the transdermal penetration of drugs through chemical methods. Microneedle can penetrate the skin to form microporous channels and promote the penetration of drugs through physical methods. The combination of microneedle and new transdermal drug carriers can significantly improve the efficiency of drug penetration through the skin. The latest research on the combination of microneedle and new transdermal drug delivery vehicles is reviewed, and the development prospect of microneedle assisted new transdermal drug delivery is prospected.