Abstract: OBJECTIVE To investigate whether the clinical application of argatroban injection is reasonable. METHODS Statistical analysis of the use of argatroban injection in hospitalized cases from Second Affiliated Hospital of Soochow University from October 2018 to March 2019. RESULTS The clinical application of argatroban injection involved multiple departments and various diseases. This review found that 75.3% of the cases had unreasonable indications, 39.3% for unreasonable usage and dosage, and 8.7% for unreasonable combined medications. CONCLUSION The application of argatroban injection is generally unreasonable. There are hyper-indication and irrational use of argatroban injection. Clinicians should pay attention to avoiding risks, using argatroban injection rationally, and selecting safe, effective and economical drugs.