Abstract: OBJECTIVE To investigate effect of fucose fluoride analogue(2-fluoro-L-fucose, 2FF) on the proliferation and invasion of breast cancer MDA-MB231 cells, and to explore the possible mechanism. METHODS Lectin blot was performed to detect the expression of core fucose in breast cancer cells after 2FF treatment. CCK-8 was used to determine the effect of 2FF treatment on the proliferation of breast cancer cells. The changes of invasive abilities of breast cancer cells after 2FF treatment were determined by Transwell assay and the expression of Hippo signaling pathway-related proteins were checked by Western blotting. RESULTS 2FF suppressed core fucosylation in the breast cancer, in a dose- and time-dependent manner. After treated by 2FF, the cell proliferation and migration was significantly inhibited. The expression of pYAP, pLATS, pMST1/2 was significantly decreased in the 2FF treated cells compared with the control cells. However, the expression of YAP, LATS, MST1 had little changes. CONCLUSION 2FF inhibit the expression of core fucosylation in the breast cancer cells, and inhibit the cell proliferation and migration. The mechanism may be related to the regulation of Hippo signaling pathway.