Abstract: OBJECTIVE To study the chemical constituents and the effects on anti-hyperuricemia of extracts from Paederia scandens (Lour.) Merr.. METHODS Hypoxanthine was used to replicate hyperuricemia in mice. The serum uric acid level in mice, the level of NO in LPS-induced macrophage RAW264.7 and DPPH free radical scavenging rate of total glycosides from Paederia scandens (Lour.) Merr. were detected. The chemical constituents of the otal glycosides were identified by HPLC-LTQ/Orbitrap MS. RESULTS The extract of Paederia scandens (Lour.) Merr. significantly reduced the serum uric acid level of hypoxanthine-induced hyperuricemia mice(P<0.05 or P<0.01); The NO level in LPS-induced RAW264.7 cells was significantly suppressed by the extract of Paederia scandens (Lour.) Merr. (P<0.05 or P<0.01); The IC₅₀ of DPPH free radical scavenging was 1.4 mg·mL^-1; The chemical constituents of the total glycosides from Paederia scandens (Lour.) Merr. were mainly included genipin-1-β-D-gentiobioside, scandoside methyl ester, paederoside, geniposide, 7,8-Dihyroiridoid, scandoside, pelargonidin-3-glucoside, rutin, isoquercitrin, astragalin, delphinidin, chrysophanol, emodin, chlorogenic acid. CONCLUSION HPLC-LTQ/Orbitrap MS method was used for rapid screening of the possible active ingredients in the extracts of Paederia scandens (Lour.) Merr., which could provide a rapid and efficient methodological reference for the basic research of the anti-hyperuricemia substances of Paederia scandens (Lour.) Merr..