Abstract: OBJECTIVE To optimize the condition of preparation technique of Compound Three Herbs droplet and determine the content of ursolic acid in the droplet. METHODS Taken the hardness, roundness, trailing degrees and stickiness of the droplets as the index for evaluation, orthogonal experiment was used to optimize preparation technique of the droplet. The content of ursolic acid in the droplet was determined by HPLC. RESULTS The optimal condition of preparation technique was as follows:ratio of drug to matrix was 1:2, ratio of PEG 4000 to PEG 6000 was 2:1, temperature of drug was 80℃, infusion rate was 40 drops per minute. The content of ursolic acid measured by HPLC was 0.992 5 mg·g^-1. CONCLUSION The optimal preparation technique in Compound Three Herbs droplet is simple and feasible with good roundness of droplets, bright color, good forming and controllable quality. The method to determine the content of ursolic acid is stable and reliable, which can be used for quality control of droplets.